Cryopreservation of citrus seed via dehydration followedby immersion in liquid nitrogen
نویسندگان
چکیده
منابع مشابه
Employment of encapsulation-dehydration method for liquid nitrogen cryopreservation of ornamental plant explants propagated in vitro
In the present studies, an attempt has been made to develop a method of liquid nitrogen preservation of plant explants propagated in vitro in the laboratory of the Department of Ornamental Plants, of Agricultural University in Kraków: shoot apical and axillary meristems of Rosa ‘New Dawn’, somatic embryos of snowdrops Galanthus nivalis L. and G. elwesii Hook, and gametophyte of Phlebodium aureu...
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Human granulocytes (PMNL) were successfully cryopreserved for up to 14 months. The PMNL (1-2 X 10(7)/ml) were stored in 2-ml ampoules in the gas phase of liquid nitrogen at a temperature between -160 degrees C and -196 degrees C using dimethylsulphoxide (DMSO 10%) as cryoprotectant. Morphology and phagocytic and bactericidal capacity were best preserved by adding fetal calf serum to the freezin...
متن کاملRevival of Mammalian Sperm after Immersion in Liquid Nitrogen
1. A wide variety of procedures was used to test the motility of mammalian sperm after plunging them into liquid nitrogen at -195 degrees C. and later rapidly warming them to 35 degrees C. by plunging them into a suitable balanced and isotonic medium. 2. Using seminal fluid sperm from the same human donor, maximal numbers of motile sperm survived vitrification when the samples were (a) very fre...
متن کاملCryopreservation of Capparis spinosa Shoot Tips via Vitrification, Encapsulation Dehydration and Encapsulation Vitrification
In vitro shoot tips of Capparis spiunosa were cryopreserved using the vitrification, encapsulation/ dehydration and encapsulation vitrification techniques. In the vitrification procedure, a maximum of 70% regrwoth was obtained when shoot tips were treated with the Plant vitrification solution (PVS2) for 60 min at 0°C and plunged directly into liquid nitrogen. In encapsulation dehydration, shoot...
متن کاملEffect of Entomopathogenic Nematode Concentration on Survival during Cryopreservation in Liquid Nitrogen.
Entomopathogenic nematodes are used for biological control of insect pests. A method for improved cryopreservation of infective juvenile stage nematodes has been developed using Steinernema carpocapsae and Heterorhabditis bacteriophora. Optimum survival for both species was achieved with 12,000 infective juveniles/ml in glycerol and 7,500/ml in Ringer's solution. For S. carpocapsae, maximum sur...
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ژورنال
عنوان ژورنال: TURKISH JOURNAL OF BIOLOGY
سال: 2017
ISSN: 1300-0152,1303-6092
DOI: 10.3906/biy-1603-92